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modified boyden chamber ( pore size falcon® transwell inserts)  (Corning Life Sciences)

 
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    Structured Review

    Corning Life Sciences modified boyden chamber ( pore size falcon® transwell inserts)
    CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by <t>Boyden</t> <t>chamber</t> assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)
    Modified Boyden Chamber ( Pore Size Falcon® Transwell Inserts), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/modified+transwell+chambers/modified+boyden+chamber/pmc11881356-150-8-17
    Average 90 stars, based on 1 article reviews
    modified boyden chamber ( pore size falcon® transwell inserts) - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "C-terminal binding protein-2 triggers CYR61-induced metastatic dissemination of osteosarcoma in a non-hypoxic microenvironment"

    Article Title: C-terminal binding protein-2 triggers CYR61-induced metastatic dissemination of osteosarcoma in a non-hypoxic microenvironment

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-025-03350-6

    CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)
    Figure Legend Snippet: CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)

    Techniques Used: Migration, Incubation, Boyden Chamber Assay, Staining, Wound Healing Assay, Control, Recombinant

    Related Articles

    Migration:

    Article Title: Metabolic profiling reveals distinct metabolic alterations in different subtypes of pituitary adenomas and confers therapeutic targets.
    Article Snippet: Cultures were adjusted to a density of 1 × 105 cells/ml, and 100 μl of cell suspension was plated into each well of a 96-well plate and cultured for 0 h, 24 h or 48 h before adding 20 μl of MTS tetrazolium solution to each well with incubation for an additional 4 h. Absorbance at 490 nm was measured using an ELISA plate reader (Thermo, USA). .. Cell migration was assayed on fibronectin- and Matrigelcoated polycarbonate filters in modified Transwell chambers (Corning, USA). ..

    Article Title: Impact of SLC20A1 on the Wnt/β-catenin signaling pathway in somatotroph adenomas
    Article Snippet: Then, 20 μl MTS solution was added to each well and further incubated for 4 h. The absorbance at 490 nm of each well was measured using an ELISA plate reader (Thermo Fisher Scientific, Inc.). .. Cell migration and invasion were measured using fibronectin- and Matrigel-coated polycarbonate filters, respectively, and modified transwell chambers (Corning Inc.). .. In total, 5×10 4 GH3 cells in 200 μl F12K medium were added into the upper chambers and 600 μl medium [F12K + 2.5% FBS (cat. no. 10099-141; Gibco; Thermo Fisher Scientific, Inc.)+ 5% horse serum (cat. no. sh30074; HyClone; GE Healthcare Life Sciences] was added into lower chamber.

    Article Title: Dysregulated miR-137 and its target EGFR contribute to the progression of pituitary adenomas.
    Article Snippet: Pituitary adenomas (PAs) hypersecrete hormones or cause mass effect symptoms, with 10%–35% patients showing resistance to standard therapies.. Targeting epidermal growth factor receptor (EGFR) has significantly improved the clinical outcome in many cancers.. In this study, immunochemistry results showed that EGFR associated H-scores in 116 PA samples were higher than those in pituitary glands, and that p21, p27and Wif-1 associated H-scores were lower (P < 0.05 for all).

    Article Title: Suppression of growth, migration and invasion of highly-metastatic human breast cancer cells by berbamine and its molecular mechanisms of action
    Article Snippet: .. Tumor cell migration and invasion were measured by examining cell migration and invasion through fibronectin- and Matrigel-coated polycarbonate filters, respectively, using modified transwell chambers (Costar, Corning, Inc., Corning, NY). ..

    Article Title: Expression and Clinical Significance of MDM2 in Non-Functioning PitNETs
    Article Snippet: .. The migration and invasion of GT1-1 cells were measured using modified Transwell chambers (Corning, Corning, NY, USA) coated with fibronectin and Matrigel in 24-well culture plates (BD Biosciences, San Jose, CA, USA). ..

    Article Title: Expression and Clinical Significance of MDM2 in Non-Functioning PitNETs.
    Article Snippet: .. The migration and invasion of GT1-1 cells were measured using modified Transwell chambers (Corning, Corning, NY, USA) coated with fibronectin and Matrigel in 24-well culture plates (BD Biosciences, San Jose, CA, USA). ..

    Article Title: Impact of SLC20A1 on the Wnt/β‑catenin signaling pathway in somatotroph adenomas.
    Article Snippet: The sequences of the pGFP-c-shlenti plasmid were: Scramble, TTc Tcc Gaa cGT GTc acG T; a, cac cGa aGT aTG aca aTc TGT GGa TGc Tc; B, Gca aTG cTG TGT cTG acc TTc acT caG aG; c, aTa GGa aTc cTG TGT cTG aGG TaG TaT GT; and d, GcT TcT GcT cca ccG aaG TaT Gac aaT cT. Then, 20 μl MTS solution was added to each well and further incubated for 4 h. The absorbance at 490 nm of each well was measured using an eliSa plate reader (Thermo Fisher Scientific, Inc.). .. Cell migration and invasion were measured using fibronectin‐ and Matrigel‐coated polycarbonate filters, respectively, and modified transwell chambers (corning inc.). in total, 5x104 GH3 cells in 200 μl F12K medium were added into the upper chambers and 600 μl medium [F12K + 2.5% FBS (cat. no. 10099‐141; Gibco; Thermo Fisher Scientific, Inc.)+ 5% horse serum (cat. no. sh30074; Hyclone; Ge Healthcare life Sciences] was added into lower chamber. after 24 h, migrating cells that adhered to the lower membrane were fixed in 4% paraformaldehyde (for 30 min at 4 ̊C) and stained using hematoxylin (oriGene Technologies, inc.) for 5 min at room temperature. .. The average number of migrated cells was quantified in five randomly selected fields of view under a fluorescence microscope (Zeiss GmbH; magnification, x100) experiments were performed in triplicate.

    Modification:

    Article Title: Metabolic profiling reveals distinct metabolic alterations in different subtypes of pituitary adenomas and confers therapeutic targets.
    Article Snippet: Cultures were adjusted to a density of 1 × 105 cells/ml, and 100 μl of cell suspension was plated into each well of a 96-well plate and cultured for 0 h, 24 h or 48 h before adding 20 μl of MTS tetrazolium solution to each well with incubation for an additional 4 h. Absorbance at 490 nm was measured using an ELISA plate reader (Thermo, USA). .. Cell migration was assayed on fibronectin- and Matrigelcoated polycarbonate filters in modified Transwell chambers (Corning, USA). ..

    Article Title: Impact of SLC20A1 on the Wnt/β-catenin signaling pathway in somatotroph adenomas
    Article Snippet: Then, 20 μl MTS solution was added to each well and further incubated for 4 h. The absorbance at 490 nm of each well was measured using an ELISA plate reader (Thermo Fisher Scientific, Inc.). .. Cell migration and invasion were measured using fibronectin- and Matrigel-coated polycarbonate filters, respectively, and modified transwell chambers (Corning Inc.). .. In total, 5×10 4 GH3 cells in 200 μl F12K medium were added into the upper chambers and 600 μl medium [F12K + 2.5% FBS (cat. no. 10099-141; Gibco; Thermo Fisher Scientific, Inc.)+ 5% horse serum (cat. no. sh30074; HyClone; GE Healthcare Life Sciences] was added into lower chamber.

    Article Title: Dysregulated miR-137 and its target EGFR contribute to the progression of pituitary adenomas.
    Article Snippet: Pituitary adenomas (PAs) hypersecrete hormones or cause mass effect symptoms, with 10%–35% patients showing resistance to standard therapies.. Targeting epidermal growth factor receptor (EGFR) has significantly improved the clinical outcome in many cancers.. In this study, immunochemistry results showed that EGFR associated H-scores in 116 PA samples were higher than those in pituitary glands, and that p21, p27and Wif-1 associated H-scores were lower (P < 0.05 for all).

    Article Title: Costunolide inhibits osteosarcoma growth and metastasis via suppressing STAT3 signal pathway.
    Article Snippet: .. Invasion assay was performed using modified transwell chambers (Corning) precoated with Matrigel as described previously. ..

    Article Title: Suppression of growth, migration and invasion of highly-metastatic human breast cancer cells by berbamine and its molecular mechanisms of action
    Article Snippet: .. Tumor cell migration and invasion were measured by examining cell migration and invasion through fibronectin- and Matrigel-coated polycarbonate filters, respectively, using modified transwell chambers (Costar, Corning, Inc., Corning, NY). ..

    Article Title: Expression and Clinical Significance of MDM2 in Non-Functioning PitNETs
    Article Snippet: .. The migration and invasion of GT1-1 cells were measured using modified Transwell chambers (Corning, Corning, NY, USA) coated with fibronectin and Matrigel in 24-well culture plates (BD Biosciences, San Jose, CA, USA). ..

    Article Title: Expression and Clinical Significance of MDM2 in Non-Functioning PitNETs.
    Article Snippet: .. The migration and invasion of GT1-1 cells were measured using modified Transwell chambers (Corning, Corning, NY, USA) coated with fibronectin and Matrigel in 24-well culture plates (BD Biosciences, San Jose, CA, USA). ..

    Article Title: Impact of SLC20A1 on the Wnt/β‑catenin signaling pathway in somatotroph adenomas.
    Article Snippet: The sequences of the pGFP-c-shlenti plasmid were: Scramble, TTc Tcc Gaa cGT GTc acG T; a, cac cGa aGT aTG aca aTc TGT GGa TGc Tc; B, Gca aTG cTG TGT cTG acc TTc acT caG aG; c, aTa GGa aTc cTG TGT cTG aGG TaG TaT GT; and d, GcT TcT GcT cca ccG aaG TaT Gac aaT cT. Then, 20 μl MTS solution was added to each well and further incubated for 4 h. The absorbance at 490 nm of each well was measured using an eliSa plate reader (Thermo Fisher Scientific, Inc.). .. Cell migration and invasion were measured using fibronectin‐ and Matrigel‐coated polycarbonate filters, respectively, and modified transwell chambers (corning inc.). in total, 5x104 GH3 cells in 200 μl F12K medium were added into the upper chambers and 600 μl medium [F12K + 2.5% FBS (cat. no. 10099‐141; Gibco; Thermo Fisher Scientific, Inc.)+ 5% horse serum (cat. no. sh30074; Hyclone; Ge Healthcare life Sciences] was added into lower chamber. after 24 h, migrating cells that adhered to the lower membrane were fixed in 4% paraformaldehyde (for 30 min at 4 ̊C) and stained using hematoxylin (oriGene Technologies, inc.) for 5 min at room temperature. .. The average number of migrated cells was quantified in five randomly selected fields of view under a fluorescence microscope (Zeiss GmbH; magnification, x100) experiments were performed in triplicate.

    Invasion Assay:

    Article Title: Costunolide inhibits osteosarcoma growth and metastasis via suppressing STAT3 signal pathway.
    Article Snippet: .. Invasion assay was performed using modified transwell chambers (Corning) precoated with Matrigel as described previously. ..

    Membrane:

    Article Title: Impact of SLC20A1 on the Wnt/β‑catenin signaling pathway in somatotroph adenomas.
    Article Snippet: The sequences of the pGFP-c-shlenti plasmid were: Scramble, TTc Tcc Gaa cGT GTc acG T; a, cac cGa aGT aTG aca aTc TGT GGa TGc Tc; B, Gca aTG cTG TGT cTG acc TTc acT caG aG; c, aTa GGa aTc cTG TGT cTG aGG TaG TaT GT; and d, GcT TcT GcT cca ccG aaG TaT Gac aaT cT. Then, 20 μl MTS solution was added to each well and further incubated for 4 h. The absorbance at 490 nm of each well was measured using an eliSa plate reader (Thermo Fisher Scientific, Inc.). .. Cell migration and invasion were measured using fibronectin‐ and Matrigel‐coated polycarbonate filters, respectively, and modified transwell chambers (corning inc.). in total, 5x104 GH3 cells in 200 μl F12K medium were added into the upper chambers and 600 μl medium [F12K + 2.5% FBS (cat. no. 10099‐141; Gibco; Thermo Fisher Scientific, Inc.)+ 5% horse serum (cat. no. sh30074; Hyclone; Ge Healthcare life Sciences] was added into lower chamber. after 24 h, migrating cells that adhered to the lower membrane were fixed in 4% paraformaldehyde (for 30 min at 4 ̊C) and stained using hematoxylin (oriGene Technologies, inc.) for 5 min at room temperature. .. The average number of migrated cells was quantified in five randomly selected fields of view under a fluorescence microscope (Zeiss GmbH; magnification, x100) experiments were performed in triplicate.

    Staining:

    Article Title: Impact of SLC20A1 on the Wnt/β‑catenin signaling pathway in somatotroph adenomas.
    Article Snippet: The sequences of the pGFP-c-shlenti plasmid were: Scramble, TTc Tcc Gaa cGT GTc acG T; a, cac cGa aGT aTG aca aTc TGT GGa TGc Tc; B, Gca aTG cTG TGT cTG acc TTc acT caG aG; c, aTa GGa aTc cTG TGT cTG aGG TaG TaT GT; and d, GcT TcT GcT cca ccG aaG TaT Gac aaT cT. Then, 20 μl MTS solution was added to each well and further incubated for 4 h. The absorbance at 490 nm of each well was measured using an eliSa plate reader (Thermo Fisher Scientific, Inc.). .. Cell migration and invasion were measured using fibronectin‐ and Matrigel‐coated polycarbonate filters, respectively, and modified transwell chambers (corning inc.). in total, 5x104 GH3 cells in 200 μl F12K medium were added into the upper chambers and 600 μl medium [F12K + 2.5% FBS (cat. no. 10099‐141; Gibco; Thermo Fisher Scientific, Inc.)+ 5% horse serum (cat. no. sh30074; Hyclone; Ge Healthcare life Sciences] was added into lower chamber. after 24 h, migrating cells that adhered to the lower membrane were fixed in 4% paraformaldehyde (for 30 min at 4 ̊C) and stained using hematoxylin (oriGene Technologies, inc.) for 5 min at room temperature. .. The average number of migrated cells was quantified in five randomly selected fields of view under a fluorescence microscope (Zeiss GmbH; magnification, x100) experiments were performed in triplicate.



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    Image Search Results


    CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: C-terminal binding protein-2 triggers CYR61-induced metastatic dissemination of osteosarcoma in a non-hypoxic microenvironment

    doi: 10.1186/s13046-025-03350-6

    Figure Lengend Snippet: CtBP2 favors cell migration. A Representative images of K7M2 cell migration after 10 h incubation, as assessed by Boyden chamber assay. CtBP2-silenced K7M2 cells were stained in green, and CtBP2-overexpressing cells were stained in red before seeding (bar = 100 µm). B Estimation plot representing the relative number of migrating cells. The difference between the group means is represented on the right. C Representative images of cell migration (wound healing assay) taken at time 18 h after the wound. Control and CtBP2-silenced K7M2 cells were incubated in the presence or absence of recombinant CYR61 (1 µg/mL). The dotted rectangles outline the initial wound surface. D Quantitative evaluation of the cell migration rate. Results are expressed as box plot ( n = 15–22). An asterisk (*) indicates a statistically significant difference ( p < 0.05 vs. Control)

    Article Snippet: The cell migration rate was evaluated using modified Boyden chamber (8 μm pore size Falcon® Transwell inserts, Corning, Boulogne-Billancourt, France).

    Techniques: Migration, Incubation, Boyden Chamber Assay, Staining, Wound Healing Assay, Control, Recombinant

    VERU-111 suppresses migration and invasion of ovarian cancer cells. Effect of VERU-111 on migration (A) or invasion (B) of ovarian cancer cells using modified transwell chambers in 24-well plates. Cells were treated with vehicle or 20 nM VERU-111 for 48 or 24 hours, respectively. Representative images of control and treatment groups were captured at 20× magnification. All data are presented as the grand mean ± SEM of stained cells from at least 3 different fields. ∗∗ P < .01, ∗∗∗ P < .001, n.s., not significant.

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: VERU-111, an orally available tubulin inhibitor, suppresses ovarian tumor growth and metastasis

    doi: 10.1124/jpet.124.002298

    Figure Lengend Snippet: VERU-111 suppresses migration and invasion of ovarian cancer cells. Effect of VERU-111 on migration (A) or invasion (B) of ovarian cancer cells using modified transwell chambers in 24-well plates. Cells were treated with vehicle or 20 nM VERU-111 for 48 or 24 hours, respectively. Representative images of control and treatment groups were captured at 20× magnification. All data are presented as the grand mean ± SEM of stained cells from at least 3 different fields. ∗∗ P < .01, ∗∗∗ P < .001, n.s., not significant.

    Article Snippet: For migration assays, SKOV3 or OVCAR3 cells (3 × 10 4 ) were treated with vehicle or 20 nM VERU-111, suspended in 300 μ L serum-free RPMI 1640 medium, and added to the upper chamber of a modified transwell chamber (BD Falcon) inserted in a 24-well plate.

    Techniques: Migration, Modification, Control, Staining